Completing the Puzzle: A Cluster of Hunting Dogs with Tick-Borne Illness from a Fishing Community in Tobago, West Indies.

Eight hunting dogs were visited by a state veterinarian on the island of Tobago, Trinidad and Tobago, West Indies, as owners reported anorexia and paralysis in five of their dogs. The veterinarian observed a combination of clinical signs consistent with tick-borne illness, including fever, anorexia, anaemia, lethargy and paralysis. Blood and ticks were collected from each dog and submitted to a diagnostic laboratory for analysis. Microscopic analysis revealed a mixed infection of intracytoplasmic organisms consistent with Babesia spp. (erythrocyte) and Ehrlichia spp. (monocyte), respectively, from one dog, while a complete blood count indicated a regenerative anaemia (n = 1; 12.5%), non-regenerative anaemia (n = 4; 50%), neutrophilia (n = 3; 37.5%), lymphocytosis (n = 2; 25%), thrombocytopaenia (n = 3; 37.5%) and pancytopaenia (n = 1; 12.5%). DNA isolated from the eight blood samples and 20 ticks (16 Rhipicephalus sanguineus and 4 Amblyomma ovale) were subjected to conventional PCR and next-generation sequencing of the 16S rRNA and 18S rRNA gene for Anaplasma/Ehrlichia and Babesia/Theileria/Hepatozoon, respectively. The DNA of Ehrlichia spp., closely related to Ehrlichia canis, was detected in the blood of three dogs (37.5%), Anaplasma spp., closely related to Anaplasma marginale, in two (25%), Babesia vogeli in one dog (12.5%) and seven ticks (35%) and Hepatozoon canis and Anaplasma spp., in one tick (5%), respectively. These findings highlight the need to test both the vector and host for the presence of tick-borne pathogens when undertaking diagnostic investigations. Further studies are also warranted to elucidate the susceptibility of canids to Anaplasma marginale.

Geographic Population Genetic Structure of the New World Screwworm, Cochliomyia hominivorax (Diptera: Calliphoridae), Using SNPs.

The New World screwworm, Cochliomyia hominivorax (Coquerel 1858) (Diptera: Calliphoridae), is a serious parasite of livestock, humans, and other warm-blooded animals. It has been eradicated from the northern parts of its historical range down to the Panama-Colombian border where a permanent barrier zone is maintained. This eradication was accomplished through using the sterile insect technique (SIT). In 2016 there was an outbreak of C. hominivorax in the Florida Keys. In only six months, this pest was successfully re-eradicated using SIT, but the geographic origin of the invasion has yet to be resolved. It was previously determined that the Florida flies most likely represented a single invasion, and it was recommended that a finer-scale genetic assessment should be completed. Thus, this current proof-of-concept study aimed to develop a population genetic database using single nucleotide polymorphisms (SNPs) to reference outbreaks and potentially identify the origin of the Florida outbreak. This initial database consists of wild-caught samples from 4 geographic locations as well as laboratory colony samples that originated from 7 additional locations using a genotyping by sequencing (GBS) approach. Geographic population structuring was identified for twelve populations that clustered according to geographic location. The Florida outbreak samples appeared similar to samples from the outer Caribbean cluster which included samples from Dominican Republic and Trinidad and Tobago, however, these results will be further clarified with the replacement of laboratory colony samples with future wild-caught samples.

A field study on the occurrence of gastrointestinal nematodes in sheep over the wet and dry seasons in two West Indian Islands.

Gastrointestinal nematode (GIN) infestations are a major constraint to sheep production in the West Indies (WI). Intensive and semi-intensive management systems are most commonly employed. These islands display tropical weather patterns with wet and dry seasons. Semi-intensive farming combined with increased rainfall during the wet season has been reported to be most favourable for development and survival of GIN. This study was conducted to determine whether there was a relationship between GIN burdens in sheep with seasonality and management practices of farmers in Trinidad and Tobago (T&T). Farms were visited on a monthly basis from January to December 2017. A maximum of ten sheep, three to nine months of age, were selected from each farm. A total of 3,053 faecal samples were collected and analysed using the Modified McMaster technique. Environmental data on daily precipitation and temperature were collected from the Trinidad and Tobago Meteorological Office during the period of sampling. A mixed effects negative binomial regression model was constructed to analyse the relationship between gastrointestinal nematode faecal egg counts (GINFEC) with season, management system and use of dewormers as fixed effects and farm as a random effect. Average diurnal temperatures of T&T fluctuated between 23.2°C to 32.6°C and 23.9°C to 32.3°C in the dry and wet seasons, respectively. Average daily precipitation ranged between 1.6-1.8 mm and 6.1-8.5 mm during the dry and wet seasons, respectively. A decreased risk of 0.34 (95% CI 0.25-0.47, p < .0001) for GINFEC was observed in dewormed animals versus not dewormed. Semi-intensive management systems experienced an increased risk of GINFEC by 1.39 (95% CI 1.05-1.84, p = .021) compared with intensive and the risk of GINFEC was reduced by 0.40 (95% CI 0.33-0.49, p < .0001) in the wet season versus the dry. A higher prevalence of GIN was observed during the drier months of the year. Climatic conditions observed in T&T remained favourable for GIN survival, development and distribution year-round. Proper management is therefore required for reducing the occurrence of GIN in sheep of T&T throughout the year. This is the first reported study in the WI on the influence of seasonality and management on GIN infestations in sheep during the dry and wet seasons. Further investigation is needed to elucidate why GIN burdens appear to be higher in the dry season than the wet season. This study can be used as a baseline for public education in T&T as well as other developing countries.